Imaging

“A picture is worth 1000 words”
Confusius
“A picture is worth 1000 datas
the CHICS

PRESENTATION

The imaging facility offers state-of-the-art infrastructure and expertise to meet your microscopy needs. We offer a range of microscopes from conventional to confocal microscopy.

We offer slide scanning services, training in the autonomous use of equipment and image analysis. We also foster close collaborations for in-depth analysis or the development of artificial intelligence analysis.

services

The imaging department offers :

Advice to optimize the image quality of your markings.

Assistance and training in instrument use.

Slide scanning services.

Image analysis and training.

Collaborative work.

equipments

Zeiss – AxioObserver.Z1

Wide Field microscope: bright field, polarized light and fluorescence

Fluorescence: 4 parameters

DAPI*(Ex=365nm; Em=445nm)

FITC* (Ex=470nm; Em=525nm)

TRITC* (Ex=545nm; Em=605nm)

Cy5* (Ex=640nm; Em=690nm)

*or Compatible Fluorochromes

Leica Stellaris 5
Confocal Laser scanning microscope

3D imaging of thick specimens up to100µm

Excitations : 405nm, 448nm, white laser from 485nm to780 nm

5 detectors with adjustable spectral detection bands.

Easy imaging of 5 fluorochromes, (>10 possible).

Exemple of compatible fluorochromes :

DAPI + Alexa 488, 594, 647, 750
DAPI,+ Opal 520, 620, 690, 780
Almost any fluorochrome can be used, however combinaisons of fluorochromes have to be choosen carefuly.

Scanner Zeiss – AxioScan.Z1

Slide scanner – Capacity 100 slides – Bright field and fluorescence

Fluorescence: 5 parameters

DAPI* (Ex=365nm; Em=445nm)

FITC* (Ex=470nm; Em=525nm)

TRITC* (Ex=545nm; Em=605nm)

Cy5* (Ex=640nm; Em=690nm)

Cy7* (Ex=735nm; Em=809nm)

*or Compatible Fluorochromes

Scanner spectral Akoya – PhenoImager HT

80 slides capacity – Bright field and fluorescence

Demixing and tissue autofluorescence substraction

Fluorescence: 7 parameters

DAPI* (Ex=391nm; Em=453nm)

Opal480* (Ex=524nm; Em= 478nm)

Opal520* (Ex=486nm; Em=532nm)

Opal570* (Ex=538nm; Em=571nm)

Opal620* (Ex=590nm; Em=633nm)

Opal690* (Ex=699nm; Em=709nm)

Opal780* (Ex=735nm; Em=832nm)

*or Compatible Fluorochromes

SOFTWARES

Advanced image analysis software (qualitative, quantitative, supervised and unsupervised, cell segmentation, etc.) offering automation for data quantification.

Available on a computer workstation at CHICS – Free training

Visiopharm overview