CGB offers a genotyping service. CGB is provided for investigators who are unfamiliar with DNA genotyping and who need to establish a genotyping method. This protocol is designed to detect sequences in the genome by polymerase chain reaction amplification (PCR).
CGB offers a service of mycoplasma detection:
In addition, CGB provides access to the following instruments:
- Jess (Protein Simple, Biotechne): a protein analysis system
Manufacturer’s description: Jess automates traditional Western blotting while maximizing multiplexing with multiple detection channels.
Application currently used: Western blot
- Applied Biosystems 7900 HT Fast Real-Time PCR System: a classical Quantitative PCR system
Manufacturer’s description: The Applied Biosystems 7900HT Fast Real-Time PCR System is a real-time quantitative PCR system compatible with 96-well plate, 384-well plate and TaqMan low-density array (TLDA) cards. Acknowledged as the gold standard in real-time PCR, the 7900HT system combined with TaqMan®Assays enables you to achieve unprecedented throughput and flexibility, allowing you to pursue projects beyond the scope of previous real-time instruments
Application currently used: Gene expression analysis, allelic discrimination,library quantification
- QX200 Droplet Digital PCR System (Bio-Rad): a digital PCR system
Manufacturer’s description: The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.
Application currently used: Pathogen detection, Next generation sequencing, Gene expression analysis
- Applied Biosystems 3500 XL Genetic Analyzer (24-Capillary arrays): Sanger sequencing system
Manufacturer’s description: Based on one of the most widely used, widely trusted sequencing methodologies available (Sanger sequencing) the 3500 Series Genetic Analyzer is designed to deliver the accuracy you demand. The 3500 platform can run a wide variety of applications, including de novo sequencing and resequencing (mutational profiling), microsatellite analysis, MLPA, AFLP, LOH, MLST, and SNP validation or screening.
Applications currently used: Sanger sequencing (read length up to 1000bp)
- NAICA System 6-color dPCR:
Reservation
CGB equipment reservations are done by OpenIRIS system, which can be accessed at the following address : https://su.openiris.io/
The use of OpenIRIS requires the creation of a personal account, please contact the CGB staff in charge to create and validate your personal account
Partners
CGB is a member of Omique core facilities network.
Contacts
Localisation
Staircase F, 2nd floor
The CGB core facility is certified Iso9001:v2015 and NFX-50-900 and it is available to all CRC members, to members of its associated Universities as well as to public and private external users.
Please, contact the CGB core facility for information and details on how to access it.