| Equipment | Staff in charge | Description and resources |
|---|---|---|
| Laser Microdissection Leica AS-LMD | Christophe KLEIN | Dissection of specific subpopulations of tissue cells under direct microscopic visualization |
Cryostat Leica CM 305S | Kevin GARBIN | Frozen block sectioning. Sections from 5µm thickness |
Microtome Leica RM2145 | Kevin GARBIN | Paraffin block sectioning. Sections from 3µm thickness |
Paraffin embedding Leica ASP300 and HistoCore Arcadia | Kevin GARBIN | Automated Dehydration and impregnation of fixed and bon-fixed tissue samples. |
Autostainer Roche Ventana Discovery XT | Kevin GARBIN | Capacity of 30 (frozen or paraffin) slides that can be treated independently for IHC, IF, FISH application. |
| Autostainer DAKO 48L | Kevin GARBIN | Capacity of 48 (frozen or paraffin) slides that can be treated independently for IHC, IF, FISH application. unmasking and dewaxing treatments must be done externally prior to use the equipment. A TintoRetriever Digital Pressure Cooker with Thermometer is available to perform unmasking and dewaxing |
| Autostainer Leica Bond RX | Kevin GARBIN | Automated autostainer suitable for IHC, ISH, FISH, multiplexing experiments. Slides can be treated independently |
| Equipment | Staff in charge | Description and resources |
Confocal Microscope Zeiss LSM 710 | Christophe KLEIN | 6 excitation wave length: 405, 458, 488, 514, 561 et 633nm. Spectral unmixing: detects up to 10 parameters simultaneously. Application |
Fluorescence microscope Zeiss Axiovert 200M | Christophe KLEIN | Equiped with 4 detection filters: DAPI (Ex=365nm; Em=445nm), FITC (Ex=470nm; Em=525nm), TRITC (Ex=545nm; Em=605nm), Cy5 (Ex=640nm; Em=690nm) Application |
| Slide Scanner Zeiss Axio Scan Z1 | Christophe KLEIN | Digitalization x10, x20, x40Automated brightfield and multichannel fluorescence (up to 5 biomarkersk, or 4 biomarkers nuclei staining)coupled with Zen software Image analysis can be performed with Visiopharm software (-cell counting, measure of areas of cells…). Analysis workstations available. |
| Laser Microdissection Leica AS-LMD | Christophe KLEIN | Dissection specific subpopulations of tissue cells Under direct microscopic visualization |
| Spectral microscope Perkin Elmer Mantra | Christophe KLEIN | Can acquire um to 7 fluorochromes or 4 immunohistochemical stains. Distinctly differentiate autofluoresence. Perkin Elmer InForm worstation allows automatic cell recognition and couting function of their phenotype. Application |
| ELISPOT CTL Immunospot | Estelle DEVEVRE | Enzymatic and fluorescence detection up to 2 colors Application |
| Equipment | Staff in charge | Descritopn and resources |
|---|---|---|
| Multi-analyte assay Luminex FlexMap3D | Hélène FOHRER-TING Estelle DEVEVRE | Rapid measurment of maximum 500 analytes per sample simultaneously with 96 and 384 well plates. Application |
| Bench top cytometer BD LSR II | Hélène FOHRER-TING Estelle DEVEVRE | 10 parameters on 3 lasers. Compatible with 12x75mm polystyrene FACS tubes. ref 352054.Complete Description Optics Application |
| Bench-top cytometer BD Fortessa X20 | Hélène FOHRER-TINGEstelle DEVEVRE | 18 parameters on 4 lasers. Compatible with 12x75mm polystyrene FACS tubes (ref 352054). Complete description Optics Application |
| FACS cell sorter BD FACS Aria III | Hélène FOHRER-TING Estelle DEVEVRE | 16 parameters on 4 lasers. Sort up to 4 populations simultaneouslyComplete description Optics Application |
FACS cell sorter BD Influx | Hélène FOHRER-TING Estelle DEVEVRE | 17 parameters on 4 lasers. Can sort up to 4 populations simultaneously. Compatible with 12x75mm polypropylene FACS tubes (Ref. 352063)Complete description Optics Application |
Automated magnetic cell sorter Miltenyi Biotec Automacs Pro | Hélène FOHRER-TING | Fully automated cell labeling, and magnetic cell sorting. Can program up to 6 independent sorting. Doubled column capacity compared to manual selection. Application |
| TECHNOLOGY | OPERATOR | DESCRIPTION AND RESSOURCES |
|---|---|---|
Multi-analyte assay Luminex | Hélène FOHRER-TING Estelle DEVEVRE | More sensitive than ELISA, Luminex technology is the right choice. When no satisfying commercial solution is available, we can help you set-up an assay. |
Flow Cytometry | Hélène FOHRER-TING Estelle DEVEVRE | We can provide you with a complete flow cytometry data analysis service . From raw data to final figures for publication Softwares available: DIVA, Flow Jo, Spice, R Application |
| Cell Imaging | Christophe KLEIN | We can provide you with a complete image analysis service. From raw data to final figures for publication Softwares available: Image J, Visopharm, Zen |